Biotium货号41001，产品描述：GelRedTMNucleicAcidGelStain\'\'3Xinwater\'\'4.0L；Biotium货号41002，产品描述：GelRedTMNucleicAcidGelStain\'\'10\'\'000XinDMSO\'\'0.5ml；Biotium货号41003，产品描述：GelRedTMNucleicAcidGelStain\'\'10\'\'000Xinwater\'\'0.5ml；Biotium货号41004，产品描述：GelRedTMNucleicAcidGelStain\'\'10\'\'000Xinwater\'\'0.5ml；Biotium货号41005，产品描述：GelGreenTMNucleicAcidGelStain\'\'10\'\'000Xinwater\'\'0.5ml；Biotium货号21002，产品描述：LumiteinTMProteinGelStain100X\'\'2ml；Biotium货号21001，产品描述：LumiteinTMProteinGelStain1X\'\'200mlProductName:GelRedTMNucleicAcidGelStain,10,000XinwaterProductProtocolCatelogNumber:41003UnitSize:0.5mLAvailability:InStockPricingInformation:UnitPrice(1-4Units)UnitPrice 1234.8(5-24Units)UnitPrice 1173.06Description:GelRedTMisanultrasensitive,extremelystableandenvironmentallysafefluorescentnucleicaciddyedesignedtoreplacethehighlytoxicethidiumbromide(EB)forstainingdsDNA,ssDNAorRNAinagarosegelsorpolyacrylamidegels.GelRedisfarmoresensitivethanEBwithoutrequiringadestainingstep(Figure1).GelRedandEBhavevirtuallythesamespectra(Figure3),soyoucandirectlyreplaceEBwithGelRedwithoutchangingyourexistingimagingsystem.GelRedTMcanbeusedtostaindsDNA,ssDNAorRNAinagarosegelsviaeitherprecastorpostgelstaining.GelRedcanalsobeusedtostaindsDNA,ssDNAorRNAinpolyacrylamidegelsviapostgelstaining.GelRedisalsocompatiblewithdownstreamDNAmanipulationssuchasrestrictiondigest,sequencing,andcloning.AseriesofsafetytestshaveconfirmedthatGelRedTMisnoncytotoxic,nonmutagenicandnonhazardousatconcentrationswellabovetheworkingconcentrationsusedingelstaining.Asaresult,GelRedcanbesafelydisposedofdownthedrainorinregulartrash,providingconvenienceandreducingcostinwastedisposal.Fordetailedtestresults,youmaydownloadacompleteGelRed/GelGreenSafetyReporthere.BiotiumoffersGelRedTM10,000Xsolutioninwater,ourlatestformulationthateliminatesthehazardsofhandlingDMSOforbettersafety.WecontinuetoofferGelRedTM10,000XsolutioninDMSO(cat#41002)forestablishedusersofGelRedTMinDMSOwhodonotwishtochangetheirlaboratoryprotocols.TheperformanceandstabilityofGelRedTM10,000XiscomparableinboththewaterandDMSOformulations.Foryourconvenience,wealsoofferready-to-useGelRedTM3Xinwater(cat#41001)thatcanbedirectlyusedforpostgelstaining.Forcustomerswholookforlargepacksize,weofferacost-savingbulkpacksizeof10mL(cat#41003-1).FEATURESSaferthanEBShownbytheAmestestandotherteststobenonmutagenicandnoncytotoxicEasydisposalPassedenvironmentalsafetytestsfordirectdisposaldownthedrainorinregulartrashUltra-sensitiveMuchmoresensitivethanEtBrandSYBRSafeExtremelystableAvailableinwater,stableatroomtemperatureforlong-termstorageandmicrowavableSimpletouseVerysimpleproceduresforeitherprecastandpostgelstainingPerfectlycompatiblewithastandardUVtransilluminatorGelRedreplacesEtBrwithnoopticalsettingchange;GelGreenreplacesSYBRorGelStarwithnoopticalsettingchange(seeFigure3)PerfectlycompatiblewithdownstreamapplicationsCompatiblewithdownstreamDNAmanipulationssuchasrestrictiondigest,cloning,andsequencing.TheMostSensitiveandStablePrecastGelStainFigure1.GelRedTMissignificantlymoresensitivethanethidiumbromide(EB)fordetectinglow-levelDNA,especiallyinthelowermolecularweightarea.Shownleftaretwo-foldserialdilutionsof1KbPlusDNALadderfromInvitrogenelectrophoresedon1%agarosegelsprecastedwithGelRedorEBin1xTBE.ThetotalamountofDNAloadedperlanewas:200ng,100ng,50ngand25ngfromlefttoright.Gelswereimagedusing300-nmtransilluminationandphotographedwithanEBfilterandPolaroid667black-and-whiteprintfilms.TheMostSensitiveandStablePostGelStainFigure2.GelRedTMdisplaysconsistentlysuperiorsensitivityforpostgelstaining,regardlessofthefilterused(Avs.C)andstorageandhandlingcondition.SYBRGold,however,showedcomparableperformanceonlywhenusedfreshfromthemanufacturerandwithaSYBRfilter(Bvs.D).Followingafewfreeze-thawcycles,SYBRGold10,000Xsolutiondegradedsignificantly,resultinginpoorstaining(E).SYBRGold1Xsolutionalsodegradesovertime(seeFigure4).Two-foldserialdilutionsof1kbPlusDNALadderfromInvitrogenwereelectrophoresedon1%agarosegelsin1xTBEandpost-stainedwithGelRedTMandSYBRGold,respectively.Gelswereimagedusing300-nmtransilluminationandphotographedwiththeindicatedfiltersandPolaroidblack-and-whiteprintfilms.ThetotalamountofDNAperlaneforeachserialdilutionwas:200ng,100ng,50ngand25ngfromlefttoright.Figure3.ExcitationandemissionspectraofGelRedandGelGreeninthepresenceofDNAinPBSbufferNote:*GelRedanditsusesarecoveredbypendingUSandinternationalpatents.**SYBRistrademarkofMolecularProbes,Inc.andGelStaristrademarkofFMCcorporation.PleasealsoseeourEvaGreenTM(cat#31000),abreakthroughnucleicaciddyeideallysuitedforquantitativereal-timePCR(qPCR).Byincorporatingasmart\"release-on-demand\"DNA-bindingtechnology,EvaGreenTMhaslowPCRinhibitionwhileexhibitingsuperiorsensitivity.SimilartoourGelRedTM,EvaGreenTMhasremarkablestability.Reference:1.Liu,M.,etal.Label-freefluorescentdetectionofCu(II)ionsbasedonDNAcleavage-dependentgraphene-quenchedDNAzymes.Chem.Commun.DOI:10.1039/c1cc12006a(2011).ForElectrophoreticMobilityShiftAssay:1.Liu,Y.,etal.Biochemistry,DOI:10.1021/bi902050p,2010,2.Konate,K.,etal.Biochemistry,DOI:10.1021/bi901791x,2010.美国Biotium公司是一家在荧光技术领域独树一帜的生物技术公司，公司位于美国加利福尼亚的BAYAREA------世界上首要的生物科技中心。该公司一直致力于开发和生产用于生物医学研究的荧光指示剂及其它特殊生化产品，如Fluo-3\'\'Fura-2\'\'BCECF，JC-1等。其产品被广泛应用于细胞生物学、分子生物学、免疫学、微生物学、诊断学、生物化学、神经科学、血液流动检测及大规模筛选等众多领域。美国Biotium公司产品线BiotiumoffersavarietyoffluorescentreagentsandrelatedbiochemicalsthatfindapplicationsinlifescienceresearchanddrugdiscoveryincludingHighThroughputDrugScreening(HTS).荧光试剂:FluorescentCalciumIndicatorsandRelatedProducts(钙离子测定试剂及其衍生产品)FluorescentCl\'\'pHandZincIndicators（其他荧光离子探针（pH\'\'Cl））FluorescentMembraneandMembranePotentialDyes(生物膜及生物膜电位差荧光染料)NaturalProductsandTheirFluorescentConjugates(生物毒素及它们的荧光偶合物)BiochemicalReagentsforNitricOxide(NO)Studies(用于NO研究的生化试剂)EnzymeSubstrates(生物酶底物)ReactiveDyesandRelatedReagents(具有Thiol反应活性的荧光染料)BiotinDerivativesNucleicAcidStainsandProductsforGenomicsStudies(核酸染色剂及基因研究试剂)CytosolicTracersandRelatedProductsProteinConjugatesProteomicsproducts(蛋白质研究试剂)生物试剂盒：ApoptosisAssaysViability/CytotoxicityAssays(细胞存活率和细胞毒性检验试剂盒）ReporterGeneAssays(荧光素酶报告基因检测)CellBiologyAssaysqPCR(实时定量PCR试剂盒)